This second edition provides a user-friendly, up-to-date handbook of reliable techniques optimized for molecular biologists. It covers the breadth of relevant established immunochemical methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including antibodies, fusion proteins and their various conjugates. ...
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This second edition provides a user-friendly, up-to-date handbook of reliable techniques optimized for molecular biologists. It covers the breadth of relevant established immunochemical methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including antibodies, fusion proteins and their various conjugates. Prominence is given to the production of antibody chains in filamentous phage.
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