Natural Products Isolation

This volume presents techniques for the extraction and purification of natural products from all biological materials. It opens with methods for approaching the isolation and preparation of the intial extract. Methods for the purification of isolated products follow, including chromatography column, HPLC, ion-exchange chromatography, and crystallization final stages. Protocols for developing the purified product into a useable form are then discussed including desalting, drying and freeze-drying techniques. The final ... Read More

This volume presents techniques for the extraction and purification of natural products from all biological materials. It opens with methods for approaching the isolation and preparation of the intial extract. Methods for the purification of isolated products follow, including chromatography column, HPLC, ion-exchange chromatography, and crystallization final stages. Protocols for developing the purified product into a useable form are then discussed including desalting, drying and freeze-drying techniques. The final section of the book concerns the identification and analysis of the extracted product utilizing chemical tests. Special attention is given to the analysis of water soluble products, special problems associated with extracting plants, and large scale extraction. The last two chapters cover the development of the extracted product into a commercially usable form and the random isolation of microbial metabolites. This manual elucidates all the relevant techniques and issues addressed by researchers involved in the isolation and purification of natural methods. Read Less